Improved in vitro endothelialization on nanostructured titania with tannin/glycosaminoglycan-based polyelectrolyte multilayers

Blood compatibility of cardiovascular implants is still a major concern. Rapid endothelialization these implant surfaces has emerged as promising strategy to enhance hemocompatibility and prevent complications such thrombus formation restenosis. The successful mostly depends on the migration endothelial cells (ECs), differentiation stem cells, inhibition smooth muscle cell (SMC) proliferation. Our previous study demonstrated that nanostructured titania modified with polyelectrolyte multilayers based tanfloc (a cationic tannin derivative) glycosaminoglycans (heparin hyaluronic acid) have improved antithrombogenic properties. In this work, we used in vitro culture ECs SMCs investigate outcomes surface modifications endothelialization. were seeded surfaces, their viability, adhesion, proliferation evaluated after 1, 3, 5 days. Indirect immunofluorescent staining was determine cellular expression through presence specific marker proteins 7 10 days, EC NT also investigated. heparin showed enhanced proliferation, migration. However, SMC not promoted by surfaces. Therefore, may promote without stimulating which could improve enhancing risk leading modification here proposed candidate be applications due